Beta-Lactam ELISA Test Kit
| Unit Price: | Get Latest Price |
|---|---|
| Payment Type: | T/T |
| Incoterm: | FOB |
| Min. Order: | 1 Bag/Bags |
| Delivery Time: | 7 Days |
| Transportation: | Ocean,Land |
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Product Description
- Model No.: RND99027
- Packaging: color box
- Brand Name: reagen
- Place of Origin: usa
- Supply Ability: 500kit/month
Product Description
REA GEN ™ Beta-Lactam ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of beta-lactams including ampicillin, penicillin G, amoxicillin, cloxacillin, oxacillin in milk, urine, tissue (liver, kidney, and muscle), serum, and plasma samples. REA GEN ™ Beta-lactam ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect beta-lactam containing antibiotics to satisfy customer concerns about food safety.
The unique features of the kit are:
High recovery (75-105%), low-cost rapid sample prep protocol (10 minutes - 1 hour)
High sensitivity: 0.4 ng/gram = 0.4 ppb (parts per billion).
High reproducibility (interplate and intraplate CV typically <8%)
Rapid assay protocol (less than 2 hours to process 40 samples in duplicate)
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest (beta-lactam antibiotic) has been coated in the plate wells. The sample to be tested is mixed with a high-affinity beta-lactam capture protein and added to the well. If the beta-lactam target is present in the sample, it will compete for the capture protein, thereby preventing the capture protein from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the capture protein bound to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. The levels of beta-lactam antibiotic in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the beta-lactam standard provided in the kit.
Kit Contents, Storage and Shelf Life
|
Kit Contents |
Amount |
Storage |
|
Beta-Lactam Coated Plate |
1 x 96-well Plate (8 wells x 12 strips) |
2-8°C |
|
Penicillin G powder stock (1000 ng): Empty Vials for Standards: Negative control (white cap tube) 0.04 ng/mL (yellow cap tube) 0.08 ng/mL (orange cap tube) 0.2 ng/mL (pink cap tube) 0.3 ng/mL(purple cap tube) 0.6 ng/mL (blue cap tube) |
2
1 1 1 1 1 1 |
2-8°C * |
|
10X Beta-Lactam Capture Protein |
1.8 mL |
2-8°C * |
|
TMB Substrate ** |
10 mL |
|
|
200X HRP-Conjugated Antibody #2 |
100 mL |
|
|
Antibody #2 Diluent ** |
20 mL |
|
|
Stop Buffer ** |
20 mL |
|
|
20X Wash Solution ** |
30 mL |
|
|
10X Beta-Lactam Extraction Buffer* Milk Standard Dilution Buffer 1 |
2 x 28 mL 15 mL |
* If the kit is to be stored for over two weeks before use, store Standard Dilution Buffer1, Penicillin G powder stock, 10X Beta-Lactam Capture Protein and 100X HRP-Conjugated Antibody #2 at -20°C in a freezer that does not cycle to higher temperatures (i.e. a non-frost-free freezer). To avoid repeated freeze-thawing of the capture protein it is strongly recommended to store this component in single-use aliquots.
Sensitivity
|
Antibiotic |
Milk, Meat, Tissue, Fish and Urine(ppb) |
Serum and Plasma(ppb) |
|
Penicillin G |
0.40 |
0.80 |
|
Ampicillin |
0.43 |
0.86 |
|
Azlocillin |
0.40* |
0.80 |
|
Pipercillin |
0.52 |
1.04 |
|
Nafcillin |
0.58 |
1.16 |
|
Amoxicillin |
0.62 |
1.24 |
|
Cloxacillin |
0.64 |
1.28 |
|
Oxacillin |
0.64 |
1.28 |
|
Cefoperazone |
0.67 |
1.34 |
|
Ceftiofur |
0.7 |
1.4 |
Specificity
|
Analytes |
Cross-Reactivity (%) |
|
Penicillin G |
100 |
|
Ampicillin |
92 |
|
Azlocillin |
112 |
|
Piperacillin |
70 |
|
Nafcillin |
55 |
|
Amoxicillin |
44 |
|
Cloxacillin |
40 |
|
Oxacillin |
40 |
|
Cefoperazone |
34 |
|
Ceftiofur |
25 |
China
Min. Order:1
China
Min. Order:1ml
China
Min. Order:1kg
China
Min. Order:1kg
REAGEN LLC for sensitive, specific quantitation of a wide range of targets and samples, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits.Visit our website today to find more information on our test kits!
any interest please contact:
reagenllc at gmail dot comBasic Information
- Company Name:
REAGENLLC
- Business Type:
Manufacturer , Retailer
- Product/Service:
vitamin a test kit , veterinary elisa kits , elisa test kit , enzyme kit , elisa protocol , hiv test kit
- No. of Total Employees:
N/A
- Product Range:
Clinical Analytical Instruments
- Certificate:
Manufacturer , Retailer
- Company Address:
102 Commerce Dr. Unit 8
Factory Information
- No. of R&D Staff:
Fewer than 5 People
- No. of QC Staff:
Fewer than 5 People
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