Trimethoprim ELISA Test Kit
| Unit Price: | Get Latest Price |
|---|---|
| Payment Type: | T/T |
| Incoterm: | FOB |
| Min. Order: | 1 Bag/Bags |
| Delivery Time: | 7 Days |
| Transportation: | Ocean,Land |
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Product Description
- Model No.: RND99037
- Packaging: COLOR BOX
- Brand Name: REAGEN
- Place of Origin: USA
- Supply Ability: 500kit/month
- Certification: FDA
Product Description
REA GEN ™ Trimethoprim ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Trimethoprim (TMP) in fish, shrimp, feed, meat (beef, chicken and pork), milk,serum and urine.
The unique features of the kit are:
§ Rapid extraction method for various sample types
§ High recovery rates (>80%)
§ High sensitivity (0.015 ng/g or ppb)
§ Quick ELISA assay (less than 2 hours regardless of number of samples)
§ High reproducibility
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with Trimethoprim (TMP) antibody. During analysis, sample is added along with the TMP-horseradish peroxidase (TMP-HRP) conjugate. If the TMP residue is present in the sample, it will compete for the TMP antibody, thereby preventing the TMP-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the TMP residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Trimethoprim ELISA Test Kit has the capacity for 96 determinations of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
|
Kit Contents |
Amount |
Storage |
|
Trimethoprim Plate |
1x96-well Plate(8wellsx12 strips) |
2-8°C |
|
Trimethoprim Standards: Negative control (white cap tube) 0.015 ng/mL (yellow cap tube) 0.04 ng/mL (orange cap tube) 0.12 ng/mL (pink cap tube) 0.36 ng/mL (purple cap tube) 1.08 ng/mL (blue cap tube) 1000 ng/mL(spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C
2-8°C |
|
TMP-HRP Conjugate |
13mL |
2-8°C |
|
10X Sample Extraction Buffer |
25 mL |
|
|
20X Wash Solution ** |
28 mL |
|
|
Stop Buffer ** |
14 mL |
|
|
TMB Substrate ** |
12 mL |
|
|
Milk Extraction Buffer(Optional) |
25mL |
* If you are not planning to use the kit for over 1 month, store Trimethoprim standards and Trimethoprim -HRP Conjugate at -20°C or in a freezer.
Sensitivity
|
Sample Type |
Detection Limit (ng/g or ppb) |
|
Feed |
0.8 |
|
Fish/Shrimp/Meat/Liver/Kidney |
0.2 (Method I); 10 (II) |
|
Urine /Serum/ Plasma |
0.2 |
|
Milk/milk powder |
10 |
Specificity
|
Analytes |
Cross-Reactivity (%) |
|
Trimethoprim |
100 |
|
Sulfapyridine |
<0.1 |
|
Sulfamethoxypyridazine |
<0.1 |
|
Sulfadiazine |
<0.1 |
|
Sulfamoxole |
<0.1 |
|
Sulfisoxazole |
<0.1 |
|
Sulfathiazole |
<0.1 |
|
Sulfamerazine |
<0.1 |
|
Sulfaquanidine |
<0.1 |
|
Sulfadoxine |
<0.1 |
Required Materials Not Provided With the Kit
§ Microtiter plate reader (450 nm)
§ Incubator
§ Tissue Mixer (e.g. Omni Tissue Master Homogenizer)
§ 10, 20, 100 and 1000 mL pipettes
§ Multi-channel pipette: 50-300 mL (Optional)
§ N-hexane
§ Methanol
China
Min. Order:1
China
Min. Order:1ml
China
Min. Order:1kg
China
Min. Order:1kg
REAGEN LLC for sensitive, specific quantitation of a wide range of targets and samples, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits.Visit our website today to find more information on our test kits!
any interest please contact:
reagenllc at gmail dot comBasic Information
- Company Name:
REAGENLLC
- Business Type:
Manufacturer , Retailer
- Product/Service:
vitamin a test kit , veterinary elisa kits , elisa test kit , enzyme kit , elisa protocol , hiv test kit
- No. of Total Employees:
N/A
- Product Range:
Clinical Analytical Instruments
- Certificate:
Manufacturer , Retailer
- Company Address:
102 Commerce Dr. Unit 8
Factory Information
- No. of R&D Staff:
Fewer than 5 People
- No. of QC Staff:
Fewer than 5 People
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